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Once the tissue sample arrives at the lab, it undergoes a process called immunohistochemistry (IHC) or, more accurately, an IHC assay. This is where the magic happens, and the *triple-negative* status is confirmed. The lab technicians use special stains (antibodies) to test the tissue for the presence of estrogen receptors (ER), progesterone receptors (PR), and HER2 protein. If all three of these tests come back negative, then the cancer is classified as triple-negative breast cancer. It's like a checklist; if the cancer cells tick all three
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